Each fish was lightly anesthetized with buffered MS-222 (tricaine methanesulfonate; Sigma-Aldrich, St Louis, MO, USA), restrained in a mesh sling, and suspended under water in a 19-L plastic vessel. The fish was suspended so that the top of the head was approximately 6 cm below the surface of the water and 22 cm above the underwater speaker.

• Topical buffered MS-222 • Blunt force trauma to the head • Benzocaine hydrochloride ¹Confirmation of death must include secondary method such as decapitation followed by pithing, double pithing, exsanguination, or chemical methods for destroying brain function. Immersion Anesthetics (Ex: Buffered MS-222) MS-222 is a basic compound and must be buffered using sodium bicarbonate. The resulting solution must be between pH 7 and 7.5. movement. MS-222 solution should be buffered with sodium bicarbonate to a neutral pH before immersing fish. Non-buffered MS-222 is acidic and causes an aversive reaction in unanesthetized fish. • Anesthesia with tricaine methane sulfonate (MS222, 168 mg/l) followed by rapid freezing in liquid nitrogen. The MS-222 solution in this study was buffered with a clinically recommended dose of sodium bicarbonate (equal dry weight of MS-222), which was presumed to buffer the solution to a neutral pH (7.0–7.5); however, ideally the pH of the anesthetic solution should be measured prior to patient immersion to ensure adequate buffering (Wright, 2001

Buffered MS-222 treatments were neutralized with sodium bicarbonate to bring the pH of exposure water to that of rearing water (pH ∼7.0). Fish were exposed to MS-222 treatments for 10 minutes after

• Topical buffered MS-222 • Blunt force trauma to the head • Benzocaine hydrochloride ¹Confirmation of death must include secondary method such as decapitation followed by pithing, double pithing, exsanguination, or chemical methods for destroying brain function. Immersion Anesthetics (Ex: Buffered MS-222) MS-222 is a basic compound and must be buffered using sodium bicarbonate. The resulting solution must be between pH 7 and 7.5.

The amount of MS-222 needed for a given volume bath can (omitting the full set of conversion factors for sake of simplicity) be expressed as dosage (mg/liter) x volume (liters)/1,000= amount MS-222 (grams). The amount of sodium bicarbonate needed for a given bath can be expressed as amount MS-222 x 2= amount sodium bicarbonate.

MS-222 is a basic compound and must be buffered using sodium bicarbonate. The resulting solution must be between pH 7 and 7.5. Apr 25, 2019 · Immediately prior to dissection, the fish were euthanized using a lethal dose of buffered MS-222 (Pentair Aquatic Eco-Systems, Apopka, FL), and then weighed (g), and measured for total length (mm TL). Buffered Ms 222 Anesthetic, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more MS-222 solution should be buffered with sodium bicarbonate to a pH of approximately 7.0 because unbuffered MS-222 solution is acidic and irritating to frog skin resulting in poor absorption and unnecessary additional stress to the organisms. • Topical buffered MS-222 • Blunt force trauma to the head • Benzocaine hydrochloride ¹Confirmation of death must include secondary method such as decapitation followed by pithing, double pithing, exsanguination, or chemical methods for destroying brain function.